Coding

Part:BBa_K2765021:Design

Designed by: Jun Li   Group: iGEM18_BIT-China   (2018-09-15)

To determine whether overexpression of yno1 can induce the endogenous ROS accumulation in yeast, we constructed an expression plasmid based on pESC-Leu, in which the cloned yno1 is driven by GAL1 promoter, and thus the target gene is induced by galactose and repressed by glucose.

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Obtained gene yno1 though clone from yeast genome.

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we constructed an expression plasmid based on pESC-Leu, ligase gene yno1 into it, We use E.coli as our host cell to construct the rcombinant plasmid, then transformed it into Saccharomyces cerevisiae.

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Here shows the result of Colony PCR in E.coli.